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LANL GFP Technology

Licensing and Intellectual Property

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GFP Published Patents

US20060257942A1 Protein subcellular localization assays using split fluorescent proteins 2006-11-16

US20060257887A1 Protein -protein interaction detection system using fluorescent protein microdomains 2006-11-16

US20060252063A1 Circular permutant GFP insertion folding reporters 2006-11-09

US20050221343A1 Self-assembling split-fluorescent protein systems 2005-10-06

US20040078148A1 Directed evolution methods for improving polypeptide folding and solubility and superfolder fluorescent proteins generated thereby 2004-04-22

US20030138843A1 Method for determining and modifying protein/peptide solubility 2003-07-24

US6867042 Method for determining and modifying protein/peptide solubility 2005-03-15

US6448087 Method for determining and modifying protein/peptide solubility 2002-09-10

WO06062882C2 Protein-Protein Interaction System Using Fluorescent Protein Microdomains

WO06091638A2 Circular Permutant GFP Insertion Folding Reporters

WO06062882A3 Protein-Protein Interaction System Using Fluorescent Protein Microdomains

WO06062882A2 Protein-Protein Interaction System Using Fluorescent Protein Microdomains

WO06062877A2 Proteing Subcellular Localization Assays Using Split Fluorescent Proteins

 

Licensing Terms

Los Alamos licenses GFP on a non-exclusive basis for internal research use only. The terms of this license is dependent on which elements of the GFP toolkit are included. The various components of the toolkit are:

Component 1. An in vivo and in vitro protein tagging and detection system. It provides a fluorescent screen for proteins, gives either total expression or soluble expression information in living cells or in test tube. The fusion tag is 15 amino acids long.
        Specific materials: Split GFP 1-10 + S11 strains and plasmids

Component 2. An in vivo and in vitro protein interaction detector system. It screens for protein interactions based on GFP fluorescence. Fusion tags are ca. 15 amino acids long.
        Specific materials: Split GFP 1-9 + 10 + 11 strains and plasmids

Component 3. Tools for screening proteins (Superfolder GFP and various folding reporters and open reading frame selectors.

(a) High-throughput screen for protein folding in living cells.
Insertion type GFP: protein is inserted into GFP scaffolding. Eliminates internal ribosome binding site artifacts. Available in 4 levels of sensitivity to target protein misfolding.
        Specific materials: Set of 4 GFP insertion type vectors, plasmids and strains.

C-terminal GFP: protein is inserted in front of GFP. 

        Specific materials: C-terminal GFP fusion vector, plasmid and strain.

(b) Superfolder GFP
Version of GFP that is extremely stable, can be used in thermophiles, and is up to 50-fold brighter than eGFP fused to proteins of interest. Fusion fluorescence is proportional to total expression.

(c) Open reading frame selector based on insertion DHFR.
Proteins are inserted into the DHFR scaffolding, conferring trimethoprim resistance to E. coli. Proteins with frame shifts or stop codons are eliminated from user construct libraries.

 

For information on licensing the various components of LANL's GFP individually, all of the components together, or exclusively, please contact us.

 

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