Preparation Of Dna-Containing Extract For Pcr Amplification

The method may provide a DNA-containing extract sufficiently pure for PCR amplification within 5-10 minutes.

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Preparation Of Dna-Containing Extract For Pcr Amplification

Environmental samples typically include impurities that interfere with PCR amplification and DNA quantitation. Samples of soil, river water, and aerosol were taken from the environment and added to an aqueous buffer (with or without detergent). Cells from the sample are lysed, releasing their DNA into the buffer. After removing insoluble cell components, the remaining soluble DNA-containing extract is treated with N-phenacylthiazolium bromide, which causes rapid precipitation of impurities. Centrifugation provides a supernatant that can be used or diluted for PCR amplification of DNA, or further purified. The method may provide a DNA-containing extract sufficiently pure for PCR amplification within 5 10 minutes.

U.S. Patent No.: 7,074,565 (DOE S-99,941)

Patent Application Filing Date: May 15, 2003

Patent Issue Date: July 11, 2006

Licensing Status:

Available for Express Licensing(?). View terms and a sample license agreement.

For more information, contact Licensing@lanl.gov.


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