Flow Cytometry: High-Throughput Analysis of Samples in Flowing Liquid Using End-on Imaging and Parallel Detection
Abstract
In fluorescence-based flow cytometry, sample objects (molecules, cells, microspheres, etc.) are placed in a flowing stream of liquid that carries them through a focused laser beam, where fluorescently labeled targets are detected and analyzed serially (one by one). Throughput is limited by the rate of sample introduction, photon counting statistics, saturation of detection optics, and the overlap of sample objects in the detection volume. To overcome this problem of limited throughput, Los Alamos scientists have developed a scheme, comprising hardware and software, for the parallel analysis of many targets concurrently.
Application(s)
Los Alamos National Laboratory has developed a technology capable of increasing sample throughput and analysis for fluorescence-based flow cytometry.
Advantages
- Increases throughput by approximately 10-fold.
- Eliminates requirement for injection capillaries and hydrodynamic focusing.
- Use of large-diameter flow channel reduces problems associated with sample adhesion, sample degradation, and capillary clogging.
IP Status: Available both Exclusively and Non Exclusively
Commercialization Strategy: We are offering licenses in two categories: (i) an exclusive license to a company that will distribute the bacterial packaging cell lines individually or in kit form and (ii) nonexclusive licenses to companies that will to use the technology in providing services.
Specifie licensing terms and conditions will be negotiated on a case by case basis. For information on our licensing practices see http://www.lanl.gov/orgs/tt/license/.
Reference Number: 105
S Number: DOE reference no.(s): 91,724
Patents & Applications:
United States National Patent Number 6309886 Issued on 10/30/2001
Posted: 07-22-2009
Contact
David Hadley
Technology Transfer Division
Los Alamos National Laboratory
P.O. Box 1663, MailStop C334
(505) 667-7539
dhadley@lanl.gov
