Andrew Bradbury

Signature, Ligand & Assay Development

Andrew Bradbury was trained in medicine at the universities of Oxford and London, and subsequently practiced medicine for five years (one full time, and four part time) in the UK. He received his Ph.D. (Cambridge University) in the MRC Laboratory of Molecular Biology under the guidance of Dr. Cesar Milstein. After his Ph.D. he spent ten years in Italy: three years as a post doc in the CNR Institute of neurobiology, Rome, Italy; and seven years in Trieste, where he was first visiting professor, and subsequently tenured as assistant professor at the International School for Advanced Studies (SISSA, Trieste, Italy). He has been a technical staff member at Los Alamos National Lab since July 1999.
He has worked in the field of phage display and antibody engineering for fifteen years, and has helped organize over thirty international congresses and practical courses in this field, both in Europe and the US. He has published over eighty peer reviewed articles, including a number of reviews and commentaries on phage display and antibody engineering, and has 12 filed patents/invention disclosures. He is one of the founder members of “The Antibody Society”, and is on the editorial board of three journals.

Research Interests

• Developing high throughput affinity reagent selection and screening capabilities, and the application of such capabilities to the detection and treatment of biothreat and emerging infections.
• The application of phage antibody display methods to the study and treatment of human disease
• The development of affinity reagents based on fluorescent proteins which he believes will become the affinity reagents of choice for high throughput biology

Representative Publications

Temirov, J., Bradbury, A.R.M. and Werner, J. (2007) Surface-immobilized antibody-antigen binding studies by single molecule fluorescence imaging, SPIE In press

Goldsmith, M., Kiss, C., Bradbury, A.R.M. and Tawfik, D.S. (2007) Avoiding and controlling double transformation artifacts. Prot. Eng. Design Selection In press

Velappan, N., Sblattero, D., Chasteen, L., Pavlik, P. and Bradbury, A.R.M. (2007) Plasmid incompatibility: more compatible than previously thought? Prot. Eng. Design Selection, In press

Dai, M., Fisher, H.E., Temirov, J., Kiss, C., Phipps, M.E., Pavlik, P., Werner, J.H. and Bradbury, A.R.M. (2007) The creation of a novel fluorescent protein by guided consensus engineering, Prot. Eng. Design Selection, 20, 69-79.

Velappan, N., Martinez, J.S., Valero, R., Chasteen, L., Ponce, L., Bondu-Hawkins, V., Kelly, C., Pavlik, P. Hjelle, B. and Bradbury, A.R.M. (2007) Selection and characterization of scFv antibodies against the Sin Nombre Hantavirus nucleocapsid protein. J. Imm. Meth. 321, 60-69.

Ayriss, J., Woods, T., Bradbury, A.R.M. and Pavlik, P. (2007) High throughput screening of single chain antibodies using multiplexed flow cytometry, J. Proteomic Res. 6, 1072-1082.

Kehoe, J.W., Velappan, N., Walbolt, M., Rasmussen, J., King, D., Lou, J., Knopp, K., Pavlik, P., Marks, J.D., Bertozzi, C.R., and Bradbury, A.R.M. (2006) Using phage display to select antibodies recognizing post-translational modifications independently of sequence context. Molecular Cellular Proteomics, 5, 2350-63.

Chasteen, L., Ayriss, J., Pavlik, P. and Bradbury, A.R.M. (2006) Eliminating helper phage from phage display, Nuc. Acids Res., 34, e145.

Kiss, C., Fisher, H. Pesavento, E., Dai, M., Valero, R., Ovecka, M., Nolan, R., Phipps, L., Velappan, N., Chasteen, L., Martinez, J., Waldo, G.S., Pavlik, P. and Bradbury, A.R.M. (2006) Antibody binding loop insertions as diversity elements. Nuc. Acids Res., 34, e132.

Sblattero, D., Maurano, F., Mazzarella, G. Rossi, M., Auricchio, S., Florian, F., Ziberna, F., Tommasini, A., Not, T., Ventura, A., Bradbury, A., Marzari, R. and Troncone, R. (2005) Characterization of  the anti tissue transglutaminase antibody response in NOD mice. J. Immunology, 174, 5830-6.

Di Niro, R., Ferrara, F., Not, T., Bradbury, A., Chirdo, F., Marzari, R. and Sblattero, D. (2005) Characterizing monoclonal antibody epitopes by filtered gene fragment phage display. Biochemical J. 12, 99-112.

Mawuenyega, K.G., Forst, C.V., Dobos, K.M., Belisle, J.T., Chen, J., Bradbury, E.M., Bradbury, A.R. and Chen, X. (2005) Mycobacterium Tuberculosis Functional Network Analysis by Global Subcellular Protein Profiling, Molecular Biology of the Cell, 16, 396-404.

Siegel, R.W., Velappan, N., Pavlik, P., Chasteen, L. and Bradbury, A. (2004) Recombinatorial cloning using heterologous lox sites. Genome Res. 14, 1119-1129.

Sblattero, D., Florian, F., Azzoni, E., Ziberna, F., Tommasini, A., Not, T., Ventura,  A. Bradbury, A. and Marzari, R. (2004) One-step cloning of anti tissue transglutaminase scFv from subjects with celiac disease. J. Autoimmunity, 22, 65-72.

Pavlik, P., Siegel, R.W., Marzari, R., Sblattero, D., Verzillo, V., Collins, C., Marks, J.D. and Bradbury, A. (2003) Predicting antigenic peptides suitable for the selection of phage antibodies.  Human Antibodies, 12, 99-112.

Sepúlveda,  J., Jin, H., Sblattero, D., Bradbury, A. and Burrone, O.R. (2003) Binders based on dimerisation of immunoglobulin VH domains, J. Mol. Biol. 333, 355-65.

Zacchi, P., Sblattero, D., Florian, F, Marzari, R., and Bradbury, A. (2003) Filtering DNA for Open Reading Frames. Genome Research, 13, 980-990.

Sblattero, D., Florian, F., Azzoni, E., Zyla, T., Park, M., Baldas, V., Not, T., Ventura, A., Bradbury, A. and Marzari, R. (2002) The analysis of the fine specificity of celiac disease antibodies using tissue transglutaminase fragments. Eu. J. Biochem. 269, 1-7.

Trevisiol, C., Ventura, A. Baldas, V., Tommasini, A., Santon, D., Martelossi, S., Torre, G., Berti, I., Spanò, A., Crovella, S., Amoroso, A., Sblattero, D., Marzari, R., Bradbury, A. and Not, T.  (2002) A reliable screening procedure for coeliac disease in clinical practice.  Scan. J. Gastroenterology 37, 679-684.

Marzari, R., Sblattero, D., Macor, P., Fischetti, F., Gennaro, R., Marks, J.D., Bradbury, A. and Tedesco, F. (2002) The cleavage site of C5 from man and animals as a common target for blocking human monoclonal antibodies: in vitro and in vivo studies. Eu. J. Immunol. 32, 2773-2782 .

Siegel, R.W., Jain, R. and Bradbury, A. (2001) Using an in vivo phagemid system to identify non-compatible loxP sequences. FEBS letts 505, 467-473.

Lou, J., Marzari, R., Verzillo, V., Ferrero, F., Pak, D., Sheng, M., Yang, C., Sblattero, D. and Bradbury, A. (2001) Antibodies in haystacks: how selection strategy influences the outcome of selection from molecular diversity libraries, J. Imm Methods, 253, 233-242.

Marzari R, Sblattero D., Florian F. Tongiorgi E., Ventura A., Tommasini A.,  Not T., Bradbury A. (2001) Molecular dissection of the tissue transglutaminase autoantibody response in celiac disease, J. Imm. 166, 4170-4176.

Sblattero D., Florian, F., Not T., Ventura A., Bradbury A., Marzari R. (2000) Analysing the peripheral blood antibody repertoire of a celiac disease patient using phage antibody libraries, Human Antibodies, 9, 199-206.

Baldas, V., Tommasini, A., Trevisiol, C., Berti, I., Fasano, A., Sblattero, D., Bradbury, A., Marzari, R., Ventura, A. and Not, T. (2000) Development of a novel rapid non-invasive screening test for coeliac disease. Gut, 47, 628-31.

Siegel, R., Allen, B. Pavlik, P., Marks, J.D. and Bradbury, A. (2000)  Mass spectral analysis of a protein complex using single chain antibodies selected on a peptide target: applications to functional genomics. J. Mol Biol. 302, 285-293.

Sblattero, D. and Bradbury, A. (2000), Exploiting recombination in single bacteria to make large phage antibody libraries. Nature Biotechnolgy, 18, 75-80.

Cirino, N., Sblattero, D., Allen, D., Peterson, S. Marks, J., Jackson, P. Bradbury, A. and Lehnert, B. (1999) Disruption of anthrax toxin binding with the use of human antibodies and  competitive inhibitors, Infection and Immunity 67, 2957-2963.

Persic, L. Horn, I.R., Rybak, S. Cattaneo, A. Hoogenboom, H.R. and Bradbury, A. (1999) scFvs selected on the 57-76 p21Ras neutralizing epitope from phage antibody libraries recognize the parental protein.  FEBS lett. 443, 112-116.

Sblattero, D. and Bradbury, A. (1998) A definitive set of oligonucleotide primers for amplifying human V regions, Immunotechnology, 3, 271-8.

Persic, L., Roberts, A., Wilton, J., Cattaneo, A., Bradbury, A. and Hoogenboom, H.R. (1997)  An integrated vector system for the eukaryotic expression of antibodies or their fragments after selection from phage display libraries.  Gene 187, 9-18.

Persic, L., Roberts, A., Hoogenboom, H.R., Cattaneo, A. and Bradbury, A. (1997)  Targeting vectors for intracellular immunisation.  Gene 187, 1-8.

Marzari, R., Edomi, P., Bhatnagar, R.K., Ahmad, S., Selvapandiyan, A. and Bradbury, A. (1997) Phage display of Bacillus thuringiensis CryIA(a) insecticidal toxin. Febs lett. 411, 27-31.

Marzari, R. Sblattero, D., Righi, M. and Bradbury, A. (1997)  Extending filamentous phage host range by the grafting of a heterologous receptor binding domain.  Gene, 185, 27-33.

Bradbury, A., Persic, L., Werge, T. and Cattaneo, A. (1993) From gene to antibody: the use of living columns to select specific phage antibodies. Biotech. 11, 1565-1569.

Reviews

Taussig, M., Stoevesandt, O., Borrebaeck, C. A., Bradbury, A. R. et al., (2007). ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome. Nat. Methods 4, 13-17.

Bradbury, A.R.M. and Marks, J.D. (2004). Antibodies from phage antibody libraries. J. Imm. Methods, 290, 29-49.

Bradbury, A., Velappan, N., Verzillo, V., Ovecka, M., Chasteen, L., Sblattero, D., Marzari, R., Siegel, R. and Pavlik, P. (2003). Antibodies in proteomics II: screening and using  antibodies.  Trends in Biotech, 21, 312-317.

Sblattero, D., Lou, J, Marzari, R. and Bradbury, A. (2001) In vivo recombination as a tool to generate molecular diversity in  phage antibody libraries. J. Biotechnology,  74, 303-15.