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Email
Phone (505) 667-1002

Priya K. Dighe

Priya Dighe

Capabilities

  • Biosciences
  • Algal biofuels
  • Biosecurity
  • Vaccines and therapeutics for infectious diseases
  • Pathogen studies
  • Biological threat identification, characterization, detection/diagnosis,
  • Biological countermeasure
  • Phage display
  • Microbial biochemistry
  • HIV database
  • Agricultural and zoonotic diseases
  • Biomaterials
  • Chemical Science
  • Biological chemistry
  • Algal biofuels

Expertise

Microbiology - Bacterial culturing and DNA isolation, culture identification techniques and biochemical assays. Experienced in growing BSL2 and BSL1 anaerobic bacteria (Clostridium perfringens, Clostridium tetani and other Clostridium neighbors, excluding Clostridium botulinum).

Molecular biology

  • Provides a repository and database of BSL2 bacterial DNA  by extraction DNA extraction using Qiagen kits after growing substantial amount of cultures in specific growth media. This provides a basis to run several assays for detection of pathogenic bacteria and to determine the accuracy of the detection assays using real time PCR machines, conventional PCR thermo cyclers and gel electrophoresis.
  • Prepares phage antibody libraries, selection of binders using phage display technology,  preparation  of helper phage and competent cells, DNA transformation into E. coli,  PCR cloning and optimization, plasmid preparations, DNA and protein gel electrophoresis,  protein  purification (ion exchange, IMAC, reverse phase), dot blots, western blotting, ELISA.

Education

M.S. Microbiology, Clemson University, SC., 12/1992
Thesis: Detection of a bacteriocin, Leuconocidin D, produced by Leuconostoc mesenteroides subspecies mesenteroides, from caprine milk. (GPA 3.8)

M.S. (Part I) Microbial Science, Second Class (Grade B), University of Bombay, India, 6/1989

B.S. Microbiology, First Class (Grade A), University of Bombay, India, 4/1988

Diploma in Medical Lab Technology, First Class (Grade A),  Academy of Medical Sciences, Bombay, India, 7/1989
 

 

LANL Positions

LANS-UC Biology Research Technologist 1 Bio Science Division 7 (10), 6/2012 to 2/2013
Real time PCR assays, Swipe tests, Maintenance of Instruments for BATL (Biological agents testing laboratory), Document writing-Project funded by DHS.

Worked in a BSL2 laboratory for 3 years.

LANS-UC Biology Research Technologist 1 Bio Science Division 7 (10), 5/2006 to present
Preparation and maintenance of a repository and database of BSL2 bacterial DNA  for DHS projects..
This provides a basis to run several assays for detection of pathogenic bacteria and to determine the accuracy of the detection assays using real time PCR machines, conventional PCR thermo cyclers and gel electrophoresis.

UC Biology Technician 5 Bio Science Division 7, 8/2005 to 5/2006
Worked on 'The Stealthy Insect project' for 8 months.

Molecular Biology Technician, 9/2001 to 8/2005    
Bio Science Division (B1), Los Alamos National Laboratory, NM
Participated in several molecular biology research projects which involved amplification of phage libraries, selections using these libraries and preparation of plasmid vectors. Weekly and monthly maintenance of TECAN (ELISA robotic instrument).

Research Associate, 4/1997 to 8/1998
Theoretical Biology and Biophysics Group (T-10), Los Alamos National Laboratory, NM     
Aligned sequences of HIV and Influenza viruses at nucleotide and amino acid levels
Submitted new sequences to Gen Bank for accession numbers (research funded by NIH and NIAID)
Updated Los Alamos National Laboratory’s Genetic Sequence Database.

Research Specialist I, 9/1994 to 12/1995
Department of Biological Sciences, Clemson Universtiy, SC .
Investigated biodegradation of commercially used thermal poly-aspartate (TPA) in
environmental waste waters. Research was funded by Donlar Corporation, IL. Demonstrated microbial enzymatic activity responsible for breakdown of TPA from activated sludge. Supervised graduate students' research experiments.

Research Specialist I, 7/1993 to 9/1994
Department of Biological Sciences, Clemson University, SC.
Concentrated AP4A from blood samples obtained from Shriner's Hospital for Crippled
Children, Greenville, SC. (Attended surgery while extracting samples)
Maintained PC12 (mouse adrenal tumor) cell line

Graduate Research Assistant, 12/1990 to 12/1992
Department of Microbiology and Department of Food Science, Clemson University, SC.
Isolated dairy leuconostocs producing antimicrobial activity.

Trainee Medical Technologist, 9/1989 to 12/1989
P. J. Hinduja Hospital, Bombay, India.
Gained hands-on experience in Hematology, Microbiology, Biochemistry, Histology and Blood Bank departments (rotation through all departments).

 

Professional Societies

Member of ‘Dances of India’ a charitable organization that donates proceeds to orphanages, institutions for women, needy schools etc.

 

Awards

LANL large team distinguished performance award, 8/2006

Two spot awards: one from supervisor for excellent work, second from Bio Safety Committee for a tidy, clean compliant lab

 

Publications

Global Variation in the HIV-1 V3 Region
Priya K. Dighe, Bette T. Korber and Brian T. Foley
MS K710, Los Alamos National Laboratory, Los Alamos, NM 87545