Center for Integrated Nanotechnologies Profiles
Profiles of researchers associated with CINT
James Henry Werner
Phone (505) 667-8842
- Optics and fiber optics
- Biological assemblies
- Center for Integrated Nanotechnologies (CINT)
- Structure Measurements
- Property Measurements
- Function/Response Measurements
B.S., Applied Physics, California Institute of Technology, 1992
M.S., Applied Physics, Cornell University, 1994
Ph.D., Applied Physics, Cornell University, 1998
Technical Staff Member, 2002 - present
Postdoctoral Research Associate, 1998 - 2002
American Chemical Society
American Physical Society
Fellow, American Physical Society, 2016
Los Alamos Distinguished Patent Award, 2011
R&D 100 Award “NanoCluster Beacons” , 2011
Los Alamos Postdoctoral Distinguished Mentor Award, 2011
R&D 100 Award “3D Tracking Microscope” (2008)
Hertz Foundation Fellowship (1992-1997)
"Apparatus and method for tracking a molecule or particle in three dimensions," Werner, JH, Goodwin PM, and Lessard GA, US Patent 7,498,551 (2009).
3 Dimensional Imaging at Nanometer Resolutions," Werner, JH, Goodwin, PM and Shreve, AP, US Patent 7,675,045, (2010).
"Probe and Method for DNA Detection," Yeh, HC, Sharma, JK, Martinez, JS and Werner, JH, US Patent Application No. 20110212540, (2011).
 "Note: Time-gated 3D single quantum dot tracking with simultaneous spinning disk imaging," DeVore, M, Stich, D, Keller, A, Cleyrat, C, Phipps, M, Hollingsworth, J, Lidke, D, Wilson, B, Goodwin, P and Werner, J, Review of Scientific Instruments 86, 126102, (2015).
 "Three dimensional time-gated tracking of non-blinking quantum dots in live cells," DeVore, MS, Stich, DG, Keller, AM, Ghosh, Y, Goodwin, PM, Phippsa, ME, Stewart, MH, Cleyrat, C, Wilson, BS, Lidke, DS, Hollingsworth, JA and Werner, JH, Proc. of the SPIE 9338, 1-12, (2015).
 "Interferometric three-dimensional single molecule localization microscopy using a single high-numerical-aperture objective," Zhang, P, Goodwin, P and Werner, J, Applied Optics 53, 7415-7421, (2014).
 "3‐Dimensional Tracking of Non‐blinking ‘Giant’Quantum Dots in Live Cells," Keller, AM, Ghosh, Y, DeVore, MS, Phipps, ME, Stewart, MH, Wilson, BS, Lidke, DS, Hollingsworth, JA and Werner, JH, Advanced Functional Materials 24, 4796-4803, (2014).(Work Featured in Frontispiece)
 "Fast, super resolution imaging via Bessel-beam stimulated emission depletion microscopy," Zhang, P, Goodwin, P and Werner, J, Optics Express 22, 12398-12409, (2014). (Work among top 10 most downloaded articles for Optics Express in May 2014).
 "Enhanced 3D localization of individual RNA transcripts via astigmatic imaging," Perillo, EP, De Haro, L, Phipps, ME, Martinez, JS, Yeh, HC, Dunn, AK, Shepherd, DP and Werner, JH, Proc. of the SPIE 8950, 1-11, (2014).
 "Actin restructuring during Salmonella typhimurium infection investigated by confocal and super-resolution microscopy," Han, JJ, Kunde, YA, Hong-Geller, E and Werner, JH, Journal of Biomedical Optics 19, 016011-016011, (2014).
 "Counting Small RNA in Pathogenic Bacteria," Shepherd, DP, Li, N, Micheva-Viteva, S, Munsky, B, Hong-Geller, E and Werner, JH, Analytical Chemistry 85, 4938-4943, (2013). (Work Featured on Cover).
 "A Fluorescence Light-Up Ag Nanocluster Probe That Discriminates Single-Nucleotide Variants by Emission Color," Yeh, HC, Sharma, J, Shih, IM, Vu, DM, Martinez, JS and Werner, JH, Journal of the American Chemical Society 134, 11550-11558, (2012). (Work featured in C&E News Highlight).
 "Time-Resolved, Confocal Single Molecule Tracking of Individual Organic Dyes and Fluorescent Proteins in Three Dimensions," Han, JJ, Kiss, C, Bradbury, A and Werner, JH, ACS Nano 6, 8922–8932, (2012).
 "Photoluminescence imaging of electronic-impurity-induced exciton quenching in single-walled carbon nanotubes," Crochet, JJ, Duque, JG, Werner, JH and Doorn, SK, Nature Nanotechnology 7, 126-132, (2012).
 "Bright two-photon emission and ultra-fast relaxation dynamics in a DNA-templated nanocluster investigated by ultra-fast spectroscopy," Yau, SH, Abeyasinghe, N, Orr, M, Upton, L, Varnavski, O, Werner, JH, Yeh, HC, Sharma, J, Shreve, AP, Martinez, JS and Goodson, T, Nanoscale 4, 4247-4254, (2012).
 "A DNA-templated fluorescent silver nanocluster with enhanced stability," Sharma, J, Rocha, RC, Phipps, ML, Yeh, HC, Balatsky, KA, Vu, DM, Shreve, AP, Werner, JH and Martinez, JS, Nanoscale 4, 4107-4110, (2012).
 "Visualizing Core-shell Structure in Substituted PPV Oligomer Aggregates using Fluorescence Lifetime Imaging Microscopy (FLIM)," Peteanu, LA, Sherwood, GA, Werner, JH, Shreve, AP, Smith, TM and Wildeman, J, Journal of Physical Chemistry C 115, 15607-15616, (2011).
 "Silver nanocluster aptamers: In situ generation of intrinsically fluorescent recognition ligands for protein detection," Sharma, J, Yeh, HC, Yoo, H, Werner, JH and Martinez, JS, Chemical Communications 47, 2294-2296, (2011).
 "Time-resolved three-dimensional molecular tracking in live cells," Wells, NP, Lessard, GA, Goodwin, PM, Phipps, ME, Cutler, PJ, Lidke, DS, Wilson, BS and Werner, JH, Nano Letters 10, 4732-4737, (2010).
 "Effect of shell thickness and composition on blinking suppression and the blinking mechanism in "giant" CdSe/CdS nanocrystal quantum dots," Vela, J, Htoon, H, Chen, Y, Park, YS, Ghosh, Y, Goodwin, PM, Werner, JH, Wells, NP, Casson, JL and Hollingsworth, JA, Journal of Biophotonics 3, 706-717, (2010).
 "Photophysical characterization of fluorescent metal nanoclusters synthesized using oligonucleotides, proteins and small reagent molecules," Yeh, HC, Sharma, J, Martinez, JS and Werner, JH, Proceedings of the SPIE 7576, 7576-1 to 7576-10, (2010).
 "Formation and Stabilization of Fluorescent Gold Nanoclusters Using Small Molecules," Bao, Y, Yeh, H-C, Zhong, C, Ivanov, SA, Sharma, JK, Neidig, ML, Vu, DM, Shreve, AP, Dyer, RB, Werner, JH and Martinez, JS, The Journal of Physical Chemistry C (2010).
 "Aggregation Effects on the Emission Spectra and Dynamics of Model Oligomers of MEH-PPV," Sherwood, GA, Cheng, R, Smith, TM, Werner, JH, Shreve, AP, Peteanu, LA and Wildeman, J, The Journal of Physical Chemistry C 113, 18851-18862, (2009).
 "Formation and Dynamics of Supported Phospholipid Membranes on a Periodic Nanotextured Substrate," Werner, JH, Montano, GA, Garcia, AL, Zurek, NA, Akhadov, EA, Lopez, GP and Shreve, AP, Langmuir 25, 2986-2993, (2009).
 "Going Beyond 2D: Following membrane diffusion and topography in the IgE–Fc[Epsilon]RI system using 3-dimensional tracking microscopy," Wells, NP, Lessard, GA, Phipps, ME, Goodwin, PM, Lidke, DS, Wilson, BS and Werner, JH, Proceedings of the SPIE 7185, 7185-1 to 7185-13, (2009).
 "Probing dimerization and Intraprotein fluorescence resonance energy transfer in a far red fluorescent protein from the sea anemone Heteractis crispa," Lessard, GA, Habuchi, S, Werner, JH, Goodwin, PM, De Schryver, FC, Hofkens, J and Cotlet, M, Journal of Biomedical Optics 13, 031212-1 to 031212-7, (2008).
 "Using T7 phage display to select GFP-based binders," Dai, M, Temirov, J, Pesavento, E, Kiss, C, Velappan, N, Pavlik, P, Werner, JH and Bradbury, ARM, Protein Engineering Design and Selection 21, 413-424, (2008).
 "Increasing the resolution of single pair fluorescence resonance energy transfer measurements in solution via molecular cytometry," Werner, JH, McCarney, ER, Keller, RA, Plaxco, KW and Goodwin, PM, Analytical Chemistry 79, 3509-3513, (2007).
 "The creation of a novel fluorescent protein by guided consensus engineering," Dai, M, Fisher, H, Temirov, JP, Kiss, C, Phipps, ME, Pavlik, P, Werner, JH and Bradbury, A, Protein Engineering Design and Selection 20, 69-79, (2007).
 "A two dimensional view of the folding energy landscape of cytochrome c," Werner, JH, Joggerst, R, Dyer, RB and Goodwin, PM, Proceedings of the National Academy of Sciences 103, 11130-11135, (2006).
 "Surface-immobilized antibody-antigen binding affinity studies by single molecule fluorescence imaging," Temirov, JP, Bradbury, A and Werner, JH, Proceedings of the SPIE 6092, 60920O1 to 60920O10, (2006).
 "Single Molecule Spectroscopic Characterization of a Far-Red Fluorescent protein (HcRed) from the Anthozoa Coral Heteractis Crispa," Cotlet, M, Habuchi, S, Whitier, JE, Werner, JH, De Schryver, FC, Hofkens, J and Goodwin, PM, Proc. of the SPIE 6098, 609804-1 to 690804-11, (2006).
 "Time-resolved Detection of the One- and Two-Photon Excited Fluorescence of Single Molecules of a Folding Enhanced Green Fluorescent Protein," Cotlet, M, Goodwin, PM, Waldo, GS and Werner, JH, Proc. of the SPIE 6092, 609204-1 to 609204-10, (2006).
 "A comparison of the fluorescence dynamics of single molecules of a green fluorescent protein: one- vs two-photon excitation," Cotlet, M, Goodwin, PM, Waldo, GS and Werner, JH, ChemPhysChem 7, 250-260, (2006).
 "Site-specific dimensions across a highly denatured protein; a single molecule study," McCarney, ER, Werner, JH, Bernstein, SL, Ruczinski, I, Makarov, DE, Goodwin, PM and Plaxco, KW, Journal of Molecular Biology 352, 672-682, (2005).
 "Progress towards single-molecule DNA sequencing: A one color demonstration," Werner, JH, Cai, H, Jett, JH, Reha-Krantz, L, Keller, RA and Goodwin, PM, Journal of Biotechnology 102, 1-14, (2003).
 "Aspects of chemical recognition and biosolvation within room temperature ionic liquids," Baker, GA, Baker, SN, McCleskey, TM and Werner, JH, ACS Symposium Series: Ionic Liquids as Green Solvents 856, 212-224, (2003).
 "Fluorescence correlation spectroscopic studies of diffusion within the ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate," Werner, JH, Baker, SN and Baker, GA, Analyst 128, 786-789, (2003).
 "Single molecule nucleic acid analysis by fluorescence flow cytometry," Goodwin, PM, Ambrose, WP, Cai, H, Grace, WK, Larson, EJ, Marrone, BL, Jett, JH, Werner, JH and Keller, RA, NATO Advanced Research Workshop on Biological, Biophysical and Theoretical Aspects of Polymer Structure and Transport 87, 351-370, (2002).
 "Effects of fluorescence excitation geometry on the accuracy of DNA fragment sizing by flow cytometry," Werner, JH, Larson, EJ, Goodwin, PM, Ambrose, WP and Keller, RA, Applied Optics 39, 2831-9, (2000).
INVITED BOOK CHAPTERS
 "DNA Fragment Sizing by Flow Cytometry," Ambrose, WP, Cai, H, Goodwin, PM, Grace, KG, Habbersett, RC, Jett, JH, Larson, EJ, Werner, JH and Keller, RA, Topics in Fluorescence VI: DNA Technology (2003). JR Lakowicz, editor.