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James Henry Werner

James Werner

Capabilities

  • Biosciences
  • Flow cytometry
  • Pathogen studies
  • Biological threat identification, characterization, detection/diagnosis,
  • Single cell genomics
  • Biomaterials
  • Optical imaging and tomography of biological tissue
  • Chemical Science
  • Analytical chemistry
  • Biological chemistry
  • Physical chemistry
  • Quantum dots
  • Mass spectrometers
  • Biomarkers
  • Nanostructured quantum confined materials
  • Materials
  • Nanotechnology
  • Optics and fiber optics
  • Soft and biological materials
  • Self-assembled organic thin films and solids
  • Biological assemblies
  • Science of Signatures - Remote and Standoff Sensing
  • Image analysis
  • CINT
  • Structure Measurements
  • Function/Response Measurements

Expertise

Biophysics

Optics

Nanotechnology

Instrument Development

Education

B.S., Applied Physics, California Institute of Technology, 1992

M.S., Applied Physics, Cornell University, 1994

Ph.D., Applied Physics, Cornell University, 1998

 

LANL Positions

Technical Staff Member, 2002 - present

Postdoctoral Research Associate, 1998 - 2002

 

Professional Societies

Biophysical Society

American Chemical Society

 

Awards

Los Alamos Distinguished Patent Award, 2011

R&D 100 Award “NanoCluster Beacons” , 2011

Los Alamos Postdoctoral Distinguished Mentor Award, 2011

R&D 100 Award “3D Tracking Microscope” (2008)

Hertz Foundation Fellowship (1992-1997)

 

Patents

 

"Apparatus and method for tracking a molecule or particle in three dimensions," Werner, JH, Goodwin PM, and Lessard GA, US Patent 7,498,551 (2009).

3 Dimensional Imaging at Nanometer Resolutions," Werner, JH, Goodwin, PM and Shreve, AP, US Patent 7,675,045, (2010).

"Probe and Method for DNA Detection," Yeh, HC, Sharma, JK, Martinez, JS and Werner, JH, US Patent Application No. 20110212540, (2011).

 

Publications

[Cotlet, M * Goodwin, PM * Waldo, GS * Werner, JH], "A comparison of the fluorescence dynamics of single molecules of a green fluorescent protein: One- versus two-photon excitation", CHEMPHYSCHEM, Vol.7, iss.1, p.250-260, JAN 16 2006.

[Werner, JH * Joggerst, R * Dyer, RB * Goodwin, PM], "A two-dimensional view of the folding energy landscape of cytochrome c", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol.103, iss.30, p.11130-11135, JUL 25 2006.

[Werner, JH * McCarney, ER * Keller, RA * Plaxco, KW * Goodwin, PM], "Increasing the resolution of single pair fluorescence resonance energy transfer measurements in solution via molecular cytometry", ANALYTICAL CHEMISTRY, Vol.79, iss.9, p.3509-3513, MAY 1 2007.

[Cotlet, M * Habuchi, S * Whitier, JE * Werner, JH * Schryver, FC * Hofkens, J * Goodwin, PM], "Single molecule spectroscopic characterization of a far-red fluorescent protein (HcRed) from the anthozoa coral Heteractis crispa", PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE), Vol.6098, 609804, 2006.

[Cotlet, M * Goodwin, PM * Waldo, GS * Werner, JH], "Time-resolved detection of the one- and two-photon excited fluorescence of single molecules of a folding enhanced green fluorescent protein", PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE), Vol.6092, 609204, 2006.

[Lessard, GA * Goodwin, PM * Werner, JH], "Three-dimensional tracking of fluorescent particles", PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE), Vol.6092, 609205, 2006.

[Temirov, J * Bradbury, A * Werner, J], "Surface-immobilized antibody-antigen binding studies by single molecule fluorescence imaging", Proceedings of SPIE-The International Society for Optical Engineering, Vol.6092, 609200, 2006.

[Dai, M. * Fisher, H.E. * Temirov, J. * Kiss, C. * Phipps, M.E. * Pavlik, P. * Werner, J.H. * Bradbury, A.R.M.], "The creation of a novel fluorescent protein by guided consensus engineering", Protein Engineering, Design and Selection, Vol.20, iss.2, p.69-79, February 2007.

[Lessard, G.A. * Goodwin, P.M. * Werner, J.H.], "Three-dimensional tracking of individual quantum dots", Applied Physics Letters, Vol.91, iss.22, 224106, 2007.

[Wells, N.P. * Lessard, G.A. * Phipps, M.E. * Goodwin, P.M. * Lidke, D.S. * Wilson, B.S. * Werner, J.H.], "Going beyond 2D: following membrane diffusion and topography in the IgE-Fc[epsilon]RI system using 3-dimensional tracking microscopy", Proceedings of the SPIE - The International Society for Optical Engineering, vol.7185, p.71850Z, 2009.

[Dai, M * Temirov, J * Pesavento, E * Kiss, C * Velappan, N * Pavlik, P * Werner, JH * Bradbury, ARM], "Using T7 phage display to select GFP-based binders", PROTEIN ENGINEERING DESIGN & SELECTION, Vol.21, iss.7, p.413-424, JUL 2008.

[Lessard, GA * Habuchi, S * Werner, JH * Goodwin, PM * De Schryver, F * Hofkens, J * Cotlet, M], "Probing dimerization and intraprotein fluorescence resonance energy transfer in a far-red fluorescent protein from the sea anemone Heteractis crispa", JOURNAL OF BIOMEDICAL OPTICS, Vol.13, iss.3, 031212, MAY-JUN 2008.

[Temirov, JP * Bradbury, ARM * Werner, JH], "Measuring an Antibody Affinity Distribution Molecule by Molecule", ANALYTICAL CHEMISTRY, Vol.80, iss.22, p.8642-8648, NOV 15 2008.

[Wells, NP * Lessard, GA * Werner, JH], "Confocal, Three-Dimensional Tracking of Individual Quantum Dots in High-Background Environments", ANALYTICAL CHEMISTRY, Vol.80, iss.24, p.9830-9834, DEC 15 2008.

[Werner, JH * Montano, GA * Garcia, AL * Zurek, NA * Akhadov, EA * Lopez, GP * Shreve, AP], "Formation and Dynamics of Supported Phospholipid Membranes on a Periodic Nanotextured Substrate", LANGMUIR, Vol.25, iss.5, p.2986-2993, MAR 3 2009.

[Sherwood, GA * Cheng, R * Smith, TM * Werner, JH * Shreve, AP * Peteanu, LA * Wildeman, J], "Aggregation Effects on the Emission Spectra and Dynamics of Model Oligomers of MEH-PPV", JOURNAL OF PHYSICAL CHEMISTRY C, Vol.113, iss.43, p.18851-18862, OCT 29 2009.

[Hsin-Chih Yeh * Sharma, J. * Hyojong Yoo * Martinez, J.S. * Werner, J.H.], "Photophysical characterization of fluorescent metal nanoclusters synthesized using oligonucleotides, proteins and small molecule ligands", Proceedings of the SPIE - The International Society for Optical Engineering, vol.7576, p.75760N, 2010.

[Sharma, J * Yeh, HC * Yoo, H * Werner, JH * Martinez, JS], "A complementary palette of fluorescent silver nanoclusters", CHEMICAL COMMUNICATIONS, Vol.46, iss.19, p.3280-3282, 2010.

[Yeh, HC * Sharma, J * Han, JJ * Martinez, JS * Werner, JH], "A DNA-Silver Nanocluster Probe That Fluoresces upon Hybridization", NANO LETTERS, Vol.10, iss.8, p.3106-3110, AUG 2010.

[Vela, J. * Htoon, H. * Chen, Y. * Park, Y.-S. * Ghosh, Y. * Goodwin, P.M. * Werner, J.H. * Wells, N.P. * Casson, J.L. * Hollingsworth, J.A.], "Effect of shell thickness and composition on blinking suppression and the blinking mechanism in giant CdSe/CdS nanocrystal quantum dots", Journal of Biophotonics, Vol.3, iss.10-11, p.706-717, October 2010.

[Bao, YP * Yeh, HC * Zhong, C * Ivanov, SA * Sharma, JK * Neidig, ML * Vu, DM * Shreve, AP * Dyer, RB * Werner, JH * Martinez, JS], "Formation and Stabilization of Fluorescent Gold Nanoclusters Using Small Molecules", JOURNAL OF PHYSICAL CHEMISTRY C, Vol.114, iss.38, p.15879-15882, SEP 30 2010.

[Wells, NP * Lessard, GA * Goodwin, PM * Phipps, ME * Cutler, PJ * Lidke, DS * Wilson, BS * Werner, JH], "Time-Resolved Three-Dimensional Molecular Tracking in Live Cells", NANO LETTERS, Vol.10, iss.11, p.4732-4737, NOV 2010.

[Sharma, J * Yeh, HC * Yoo, H * Werner, JH * Martinez, JS], "Silver nanocluster aptamers: in situ generation of intrinsically fluorescent recognition ligands for protein detection", CHEMICAL COMMUNICATIONS, Vol.47, iss.8, p.2294-2296, 2011.

[Yeh, HC * Sharma, JK * Han, JJ * Martinez, JS * Werner, JH], "Nano Cluster Beacon (NCB): A DNA-silver nanocluster probe that fluoresces upon hybridization", ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol.241, MAR 27 2011.

[Sharma, JK * Yeh, HC * Werner, JH * Martinez, JS], "Synthesis of oligonucleotide-templated fluorescent silver-nanoclusters with enhanced photochemical stability", ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol.241, MAR 27 2011.

[Peteanu, LA * Sherwood, GA * Werner, JH * Shreve, AP * Smith, TM], "Visualizing Core-Shell Structure in Substituted PPV Oligomer Aggregates Using Fluorescence Lifetime Imaging Microscopy (FLIM)", JOURNAL OF PHYSICAL CHEMISTRY C, Vol.115, iss.31, p.15607-15616, AUG 11 2011.

[Hsin-Chih Yeh * Sharma, J. * Han, J.J. * Martinez, J.S. * Werner, J.H.], "NanoCluster Beacon - A New Molecular Probe for Homogeneous Detection of Nucleic Acid Targets", Proceedings of the 2011 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS 2011), p.267-70, 2011.

[Yeh, H.-C. * Sharma, J. * Han, J.J. * Martinez, J.S. * Werner, J.H.], "A beacon of light: A new molecular probe for homogeneous detection of nucleic acid targets", IEEE Nanotechnology Magazine, Vol.5, iss.2, p.28-33, June 2011.

[Werner, JH * Goodwin, PM * Han, JJ * Wilson, BS * Lidke, DS], "3D, time-resolved molecular tracking in live cells", ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol.242, AUG 28 2011.

[Martinez, JS * Werner, JH * Yeh, HC * Sharma, J * Shreve, AP * Neidig, ML * Ivanov, S * Arachchige, I], "Characterization and application of small-molecule- and DNA-templated gold and silver fluorescent nanoclusters", ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol.242, AUG 28 2011.

[Shepherd, D.P. * Nan Li * Hong-Geller, E. * Munsky, B. * Werner, J.H.], "New tools for discovering the role sRNA plays in cellular regulation", Proceedings of the SPIE - The International Society for Optical Engineering, vol.8228, p.822808, 2012.

[Temirov, J. * Werner, J.H. * Goodwin, P.M. * Bradbury, A.], "Sizing the oligomers of Azami Green fluorescent protein with FCS and antibunching", Proceedings of the SPIE - The International Society for Optical Engineering, vol.8228, p.82280J, 2012.

[Crochet, JJ * Duque, JG * Werner, JH * Doorn, SK], "Photoluminescence imaging of electronic-impurity-induced exciton quenching in single-walled carbon nanotubes", NATURE NANOTECHNOLOGY, Vol.7, iss.2, p.126-132, FEB 2012.

[Sharma, J * Rocha, RC * Phipps, ML * Yeh, HC * Balatsky, KA * Vu, DM * Shreve, AP * Werner, JH * Martinez, JS], "A DNA-templated fluorescent silver nanocluster with enhanced stability", NANOSCALE, Vol.4, iss.14, p.4107-4110, 2012.

[Yau, SH * Abeyasinghe, N * Orr, M * Upton, L * Varnavski, O * Werner, JH * Yeh, HC * Sharma, J * Shreve, AP * Martinez, JS * Goodson, T], "Bright two-photon emission and ultra-fast relaxation dynamics in a DNA-templated nanocluster investigated by ultra-fast spectroscopy", NANOSCALE, Vol.4, iss.14, p.4247-4254, 2012.

[Yeh, HC * Sharma, J * Shih, IM * Vu, DM * Martinez, JS * Werner, JH], "A Fluorescence Light-Up Ag Nanocluster Probe That Discriminates Single-Nucleotide Variants by Emission Color", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol.134, iss.28, p.11550-11558, JUL 18 2012.

[So, W.Y. * Hong, J. * Kim, J.J. * Sherwood, G.A. * Chacon-Madrid, K. * Werner, J.H. * Shreve, A.P. * Peteanu, L.A. * Wildeman, J.], "Effects of solvent properties on the spectroscopy and dynamics of alkoxy-substituted PPV oligomer aggregates", Journal of Physical Chemistry B, Vol.116, iss.35, p.10504-10513, 20120906.

[Han, JJ * Kiss, C * Bradbury, ARM * Werner, JH], "Time-Resolved, Confocal Single-Molecule Tracking of Individual Organic Dyes and Fluorescent Proteins in Three Dimensions", ACS NANO, Vol.6, iss.10, p.8922-8932, OCT 2012.

[Crochet, JJ * Duque, JG * Werner, JH * Lounis, B * Cognet, L * Doorn, SK], "Disorder Limited Exciton Transport in Colloidal Single-Wall Carbon Nanotubes", NANO LETTERS, Vol.12, iss.10, p.5091-5096, OCT 2012.

[Werner, James * Martinez, Jennifer S. * Sharma, Jaswinder K. * Yeh, Hsin-Chih], "DNA-Templated Silver Nanoclusters that Fluoresce upon Hybridization", BIOPHYSICAL JOURNAL, Vol.104, iss.2, suppl.1, p.545A-545A, JAN 29 2013.

[Shepherd, Douglas P. * Li, Nan * Hong-Geller, Elizabeth * Werner, James H. * Munsky, Brian], "Spatio-Temporal Measurements and Modeling of Genetic Expression", BIOPHYSICAL JOURNAL, Vol.104, iss.2, suppl.1, p.552A-552A, JAN 29 2013.

[Shepherd, Douglas P. * Li, Nan * Micheva-Viteva, Sofiya N. * Munsky, Brian * Hong-Geller, Elizabeth * Werner, James H.], "Counting small RNA in pathogenic bacteria", Analytical Chemistry, Vol.85, iss.10, p.4938-4943, 2013.

[Obliosca, J.M. * Cong Liu * Batson, R.A. * Babin, M.C. * Werner, J.H. * Hsin-Chih Yeh], "DNA/RNA detection using DNA-templated few-atom silver nanoclusters", Biosensors, Vol.3, iss.2, p.185-200, June 2013.

[Hollingsworth, Jennifer A. * Werner, James H. * Htoon, Han * Piryatinski, Andrei * Schaller, Richard * Ghosh, Yagnaseni * Dennis, Allison M. * Keller, Aaron M. * Mangum, Benjamin * Hannah, Daniel C.], "Non-blinking Giant nanocrystal quantum dots: Ideal probes for real-time three-dimensional tracking", CLEO: QELS_Fundamental Science, CLEO:QELS FS 2013, p.QTu1P.3, 2013.

[Han, Jason J. * Kunde, Yuliya A. * Hong-Geller, Elizabeth * Werner, James H.], "Actin restructuring during Salmonella typhimurium infection investigated by confocal and super-resolution microscopy", Journal of Biomedical Optics, Vol.19, iss.1, p.016011, January 2014.

[Peteanu, L.A. * Sherwood, G.A. * Zewe, K. * Wildeman, J. * Werner, J.H. * Shreve, A.P.], "Using fluorescence microscopy of oligomer aggregates to understand the properties of conjugated polymers used in photovoltaic devices", Optics InfoBase Conference Papers, 2009.

[Peteanu, L.A. * Sherwood, G.A. * Wildeman, J. * Werner, J.H. * Goodwin, P.M. * Shreve, A.P.], "Effects of aggregation on the emission spectra and dynamics of electroluminescent materials", Optics InfoBase Conference Papers, 2010.

[Wells, Nathan P. * Lidke, Diane S. * Lisa Phipps, M. * Goodwin, Peter M. * Wilson, Bridget S. * Werner, James H.], "Tracking single quantum dots in three dimensions: Following cell receptor traffic and membrane topology", Optics InfoBase Conference Papers, 2009.

[Werne, James H. * Lisa Phipps, M. * Goodwin, Peter M. * Cutler, Patrick J. * Lidke, Diane S. * Wilson, Bridget S.], "Time-resolved 3D Tracking of individual quantum dot labeled proteins in live cells via confocal feedback", Optics InfoBase Conference Papers, 2010.

[Zhang, P. * Goodwin, P.M. * Werner, J.H.], "Fast, super resolution imaging via Bessel-Bea stimulated emission depletion microscopy", Optics Express, Vol.22, iss.10, p.12398-12409, 2014.

[Werner, Jim * Lessard, Guillaume * Goodwin, Peter], "Tracking single quantum dots in 3 dimensions", Optics InfoBase Conference Papers, 2007.