Los Alamos National Laboratory

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MOL PCR

A nucleic acid based assay chemistry that uses microsphere arrays and flow/image cytometry for the detection of human and pathogen biomarkers. 

LAUR: 16-22527

Benefits/Features

  • Multiple Markers per Sample- (MOL-PCR) has the ability to simultaneously ask more than one question about a sample at the same time, and the ability to conduct more than one test on a sample in the same reaction.
  • Broad Array of Applications- (MOL-PCR) provides genotyping for genetic susceptibility to disease and drug response, ID and forensics, detection and diagnosis, and antibiotic/drug resistance determination.
  • Ease of Assay Design- Multiplexed ligation enables simultaneous detection of diverse signatures. The design of this technology allows for universal PCR, fewer probes to design.
  • High Level of Multiplexing- High information content is collected for every sample that is processed.
  • Amenable for use with both DNA and RNA Pathogens- Simultaneous screening for all types of pathogens.
  • Works with Degraded Samples- Detection event is ligation of probes hybridized to short target fragments.
Publications

Deshpande A and White P.S. Multiplexed nucleic acid-based assays for molecular diagnostics of human disease Expert Reviews in Molecular Diagnostics, 12(6): 645-659, 2012.

Song J., Li P.E., Gans J., Vuyisich M., Deshpande A., Wolinsky M., White P.S. Simultaneous pathogen detection and antibiotic resistance characterization using SNP-based multiplexed oligonucleotide ligation-PCR (MOL-PCR). Adv Exp Med Biol 680:455-64, 2010.

Deshpande A., Jason Gans, Steven W. Graves, Lance Green, Laura Taylor, Heung Bok Kim, Yuliya A. Kunde, Pascale M. Leonard, Po-E Li, Jacob Mark, Jian Song, Momchilo Vuyisich, and P. Scott White. A Rapid Multiplex Assay for Nucleic Acid-Based Diagnostics. Journal of Microbiological Methods, 80(2):155-163, 2010.

Patents

S-99929.000, U.S. Patent No. 7,153,656, entitled “Nucleic Acid Sequence Detection using Multiplexed Oligonucleotide PCR”, granted December 26th, 2006.

S-89684.000, U.S. Patent No. 6,287,766, entitled “DNA Polymorphism Identity Determination using Flow Cytometry”, granted on September 11, 2001.

Development Stage

TRL 5

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